Recently our SO conducted an in-house quality assurance test on the disinfectant APCF has been using for the sanitizing of the BSLII cabinets and Animal Transfer Stations (ATS). His results were a surprise to all staff as they demonstrated significant microbial resistant to the antiseptic APCF has been using.
The bacterial growth of the stock solution and the working solution both showed bacterial growth too numerous to count. Gram typing, speciation and serotyping of the organisms was not performed.
APCF staff were concerned about changing the disinfectant used in APCF because of behavioural changes to animals that may occur from the novel odour of a new disinfectant introduced into the vivarium. But APCF staff realised that the current situation of a disinfectant which actively supported microbial growth was unacceptable.
APCF senior staff have therefore implemented a new Procedure for disinfecting cages and other items transferred into BSLII cabinets or ATS. It now requires spraying the outside surfaces of the item with 80% ethanol, 5% isopropyl alcohol, before placing inside the protected area of the BSLII cabinet or the ATS and then once in the protected area wiping the wet surface with an autoclaved and one use ‘KimwipR’ (i.e. the ‘KimwipR’ must be disposed after wiping down the outside surface of the item). The new procedure can be read on the Policies and Procedures page of the InfoSite website
Floors of the vivarium will now be cleaned with scent-free VirkonR disinfectant. VirkonR has a broad spectrum of anti-microbicidal activity and it also has surfactant properties that help shift dirt from surfaces. Hence, it is an ideal floor cleaning chemical.
Antec (2003) A comparison of Virkon® versus Hypochlorite Antec (A DuPont Company) Human Health Technical Information Bulletin
Evaluation of in vitro efficacy of the disinfectant Virkon. Eur J Epidemiol. Apr;11(2):193-7
Rutala WA, Weber DJ, and the Healthcare Infection Control Practices Advisory Committee (2008) Guideline for Disinfection and Sterilization in Healthcare Facilities
van Engelenburg FA, Terpstra FG, Schuitemaker H, and Moorer WR, (2002) The virucidal spectrum of a high concentration alcohol mixture. J Hosp Infect 1(2):121-5.